Biochemistry and Molecular Biology Education. A metabolic control analysis approach to introduce the study of systems in biochemistry: the glycolytic pathway in the red blood cell: Metabolic control analysis and the glycolytic pathway. Linearization of the Bradford Protein Assay. RGBradford: Accurate measurement of protein concentration using a smartphone camera and the blue to green intensity ratio. Journal of the Brazilian Chemical Society. Smartphone for point-of-care quantification of protein by Bradford assay. de Camargo, C., Vicentini, M., Gobbi, A., Martinez, D., Lima, R.Quantifying protein concentrations using smartphone colorimetry: A new method for an established test. Linearization of the Bradford protein assay increases its sensitivity: Theoretical and experimental studies. Increased uniformity in the response of the Coomassie blue G protein assay to different proteins. Sensitivity and variability of the Bradford protein assay in the presence of detergents. Measurement of protein in cell suspensions using the Commassie brilliant blue dye-binding assay. A modified micro-Bradford procedure for elimination of interference from sodium dodecyl sulfate, other detergents, and lipids. A spectral study of the charge forms of Coomassie Blue G. Mechanism of dye response and interference in the Bradford protein assay. Application of BisANS fluorescent dye for developing a novel protein assay. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Measurement of protein using bicinchoninic acid. Protein measurement with the Folin phenol reagent. The ultraviolet absorption spectra of proteins. Mild electrical stimulation and heat shock ameliorates progressive proteinuria and renal inflammation in mouse model of Alport syndrome. Protein quantification in ecological studies: A literature review and empirical comparisons of standard methodologies. No significant difference is observed between values calculated using RGB color data and those calculated using conventional absorbance data. When using ImageJ to quantify bands one of the keys is that the area to be analyzed has to be. Then, the ratio of blue to green intensity (in the RGB scale) of samples with unknown concentrations of protein is used to calculate the protein content based on a standard curve. Hi Nicole, As Mohamed, I have always used ImageJ for quantification but from WB bands instead of PCR. After performing the assay in a microplate, a picture is taken using a smartphone camera, and RGB color data is extracted from the picture using a free and open-source image analysis software application. Taking advantage of the three forms of the Coomassie Brilliant Blue dye that exist in the conditions of the Bradford assay, this paper describes how to accurately quantify protein in samples using color data extracted from a single picture of a microplate. One of the alterations of the original method is the use of a smartphone camera as an analytical instrument. Because of its widespread, the limitations and advantages of the Bradford assay have been exhaustively reported, including several modifications of the original method to improve its performance. Amongst several other methods, the Bradford assay is one of the most used. Protein quantitation is an essential procedure in life sciences research.
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